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The Complete ONCE Ingredient List: All 23 Actives and Why Each One Is There

By the ONCE formulation desk · 2026-08-28 · oncedrink.com

Short answer

ONCE contains 23 active ingredients in one 30-serving tub at $50 a month, including 5g creatine monohydrate, 5g collagen peptides, 600mg KSM-66 ashwagandha, 500mg microencapsulated NMN, 500mg lion's mane, 500mg vitamin C, 300mg magnesium glycinate, 250mg citicoline, 200mg L-theanine, 1g Ca-AKG, 750mg TMG and 10 billion CFU Bacillus coagulans. Zero caffeine. No proprietary blends.

This is the whole label. Every ingredient, every dose, every required form. No proprietary blends, because a proprietary blend exists to stop you doing exactly what this page lets you do.

The full panel

IngredientPer servingRequired form and reason
Creatine monohydrate5 gMicronized, so it disperses in cold water. Top of the studied range.
Collagen peptides5 gHydrolysed. Paired with the vitamin C synthesis requires.
Inulin1.5 gChicory root prebiotic. Feeds the Bacillus coagulans.
Taurine1 gCellular energy support.
Calcium alpha-ketoglutarate1 gKrebs-cycle molecule. Carries about 190mg elemental calcium, declared.
Glycine1 gRoughly a third of collagen's amino acid structure. Naturally sweet.
Betaine anhydrous (TMG)750 mgMethyl donor. Pairs with the NMN.
KSM-66 ashwagandha600 mgRoot only, minimum 5% withanolides. The extract the cortisol trials used.
Lion's mane extract500 mgFruiting body, beta-glucan verified. Never mycelium grown on grain.
NMN, microencapsulated500 mgEncapsulated so it survives an acidic powder and the shelf.
Vitamin C500 mgRequired cofactor for collagen synthesis.
Magnesium glycinate300 mg elementalTrue bisglycinate, not oxide.
Citicoline250 mgStable choline form the body converts toward acetylcholine.
L-Theanine200 mgCalm focus without sedation.
Hyaluronic acid120 mgLow molecular weight, oral grade.
Vitamin D32,000 IUWater-dispersible. Paired with K2.
Vitamin K2 (MK-7)120 mcgDirects calcium correctly alongside the D3.
Zinc + Copper11 mg + 1 mgChelated, 11:1, because daily zinc depletes copper.
B-complex, all eight100%+ DV eachMethylated. Deliberately low-niacinamide.
Biotin500 mcgHeld low on purpose. Mega-doses interfere with blood tests.
Vitamin A (beta-carotene) & Vitamin E100% DVWater-dispersible.
Bacillus coagulans10 B CFUSpore form, with overage to guarantee CFU at expiry.
Iodine, Selenium, Chromium, Boron100% DV, 3 mg boronSelenium as selenomethionine.
Sodium / Potassium150 / 200 mgLight electrolytes.

The interactions are the actual product

Any contract manufacturer can put 23 things in a tub. The formula is in how they sit together.

What is deliberately not in it

How to take it

ONCE launches as a 30-serving tub. One scoop into 12 to 16 ounces of cold water, stir, drink it with breakfast. That is the entire routine, and it costs $50 a month. There is no caffeine in it, so it works at 6am or 6pm.

On testing: the spec calls for a full heavy-metals panel per lot, Prop 65 compliance, and a third-party COA per lot that can be published. Accelerated stability testing assays the moisture-sensitive actives at 0, 3 and 6 months.

Common questions

What is in ONCE?

ONCE contains 23 active ingredients per serving: 5g creatine monohydrate, 5g collagen peptides, 1.5g inulin, 1g taurine, 1g calcium alpha-ketoglutarate, 1g glycine, 750mg TMG, 600mg KSM-66 ashwagandha, 500mg lion's mane fruiting-body extract, 500mg microencapsulated NMN, 500mg vitamin C, 300mg elemental magnesium as glycinate, 250mg citicoline, 200mg L-theanine, 120mg hyaluronic acid, 2,000 IU vitamin D3, 120mcg vitamin K2 as MK-7, 11mg zinc with 1mg copper, a full methylated B-complex, 500mcg biotin, vitamins A and E, 10 billion CFU Bacillus coagulans, iodine, selenium, chromium, 3mg boron and light sodium and potassium electrolytes.

Does ONCE contain caffeine?

No. ONCE contains zero caffeine by design, so it can be taken at any hour of the day and stacks with coffee rather than competing with it.

Does ONCE use proprietary blends?

No. Every ingredient's milligram amount is printed on the label. Proprietary blends exist to conceal how little of each ingredient is present.

How do you take ONCE?

One scoop into 12 to 16 ounces of cold water, stirred, once daily with breakfast. Taking it with food matters because the formula contains fat-soluble vitamins A, D3, E and K2. ONCE launches as a 30-serving tub at $50 a month.

Why does ONCE contain no iron?

Iron competes with zinc and magnesium for absorption. Leaving it out removes that interaction, so the minerals that are in the formula are better absorbed.

One scoop. 23 actives. Full studied doses.ONCE launches as a 30-serving tub at $50 a month. Founding price held for the first group.
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These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. This article is educational and is not medical advice; consult a physician before starting any supplement.